This research investigates the relationship between ethical leadership and workplace spirituality through work enthusiasm as a mediating variable. This study uses a quantitative design to test the proposed hypothesis. Data were collected from 260 employees in an education-based agency in Malaysia through a self-administered questionnaire. Partial Least Squares Structural Equation Modelling (PLS-SEM) was used to evaluate both the measurement and the structural model. The findings support the indirect relationship between ethical leadership and workplace spirituality through work enthusiasm. Furthermore, the findings also confirm the mediating role of work enthusiasm in the relationship between ethical leadership and workplace spirituality in the proposed model. The results of the analysis show that ethical leadership practices in the daily operations of the organization are able to increase the level of work enthusiasm. The effect of this increase in motivation indirectly contributes to the strengthening of spirituality in the workplace. This study provides valuable insights to practitioners in understanding the complexity of work enthusiasm in the context of ethical leadership. The implications are that these findings can inform the development of more effective strategies and action plans to achieve organizational goals in the increasingly challenging era of globalization.