In this study, the mediating role of emotional labor in the impact of organizational climate on burnout in accommodation establishments was investigated. For this purpose, a survey was conducted for 19 five-star accommodation establishments operating in Antalya, which has the most accommodation establishments in Turkey. In the study, which was carried out with a total of 976 participants, emotional labor, which is the mediating variable, was examined with its sub-dimensions, and impact analyzes were made with its sub-dimensions. The descriptive analyzes of the research were analyzed with the SPSS program, and the structural equation model was analyzed with the AMOS program. At the end of the analysis, it was seen that the organizational climate hurt burnout. With the inclusion of emotional labor, which is a mediating variable, in the model, it has been determined that emotional effort and emotional conflict, which are sub-dimensions of emotional labor, have a partial mediation impact on the effect of organizational climate on burnout. At the end of the research, recommendations for practitioners and researchers were presented.