The aim of this study was to evaluate the effect of compassion-centered education of mothers on reducing anxiety in children aged 4-6 years. The research method was a quasi-experimental, pre-test, post-test and follow-up with the experimental and control group. The research population included all mothers with preschool children in Isfahan in 2018. Using a convenient sampling method, 30 mothers who had a child with anxiety symptoms were selected and randomly divided into two experimental and control groups (each group included 15 subjects). The experimental group received compassion-centered education for 12 sessions in group, 90 minutes per session, and the control group did not receive any interventions. Demographic information questionnaire and Spence et al Children's Anxiety Scale were used as the research tools. Data were analyzed using repeated measures analysis of variance. The results revealed a significant difference among the pre-test, post-test and follow-up scores in anxiety, and the mean scores in the post-test and follow-up stages showed reduction compared with the mean score in pre-test stage and the scores in these groups showed significant changes compared to control group (p <0.05). These results were closer to the posttest in the one-month follow-up. In general, it was found that compassion-centered education of mothers was effective on reducing the children`s anxiety, because mental health of parents and the quality of their relationship can affect the quality of their children's life.