The objective of this study is to identify the factors affecting the turnover of knowledge-basedemployeesin a comparative study ofKhorasanRegional Electric Companyand KhorasanRazaviOil Products Distribution Company. The statistical population included 77 knowledge-basedemployees of the National Oil Products Distribution Company and 125 employees ofKhorasanRegional Electric Company. The factors, affecting the employees' turnover, were identified through exploratory studies and a survey fromexperts using Delphi technique. Finally, 7 factors, 35 components, and 167 items were identified. The results of structural equation modeling showed that factors affecting the turnover of knowledge-based employees can be classified into five classes, including environmental, organizational and structural, occupational, juridical, value and ideological factors in Oil Products Distribution Company, and seven factors, including organizational and structural, occupational, juridical, value and ideological and management factors in the Regional Electric Company.