Introduction and Objective: Medical Librarianship and Medical Informatics have an interdisciplinary nature so that medical librarians and information providers use other fields of science, humanities and information technologies to train specialized staff needed in educational units, libraries, and academic/medical information centers. The present study was conducted to investigate the required competencies of librarians working in Iranian universities of medical sciences from the viewpoint of professors, library managers, and librarians. Methodology: the current research is an applied research and conducted by survey method. The population in the present study is consisted of a group of profession in the field of medical librarianship, library managers and librarians who were working in Iranian universities of medical sciences. The required data were collected using a researcher-made questionnaire. The validity and reliability of the questionnaire were confirmed by professors’ comments on its content and calculating the Cronbach’s alpha (0.97). Finally, the collected data were analyzed via the SPSS software and using both descriptive and inferential statistics (p>0.05). Results: According to the results, competencies in information seeking and competencies in medical concepts and topics were respectively the most and the least important competencies, according the experts’ point of view; competencies in management and Competencies in medical concepts and topics were ,respectively, the most and the least important competencies from the viewpoint of library managers; and Personal and Moral competencies and competencies in research were ,respectively, the most and the least important competencies from the viewpoint of librarians. Discussion and Conclusions: Considering the current changes, investigating and identifying the required competencies of the librarian can optimize the education of librarianship and medical informatics; therefore, the provided competences in this study, can be effective and useful for the professions of librarianship and the medical informatics.