The article deals with the issue of tutors’ psychological readiness for professional activity in the system of Ukrainian higher education. The concepts that are defined are psychological readiness, tutor, professional tutors training, tutor activity, competence, and innovations in the educational system; and the core activity of the Center of Excellence at Bogdan Khmelnitsky Melitopol State Pedagogical University is described. Innovative strategies for the development of the international community lead to the creation of such a system of education which will enable the growth of creative potential of the individuals, and their spiritual and moral characteristics. The cultural and educational institutions have the task to organize the educational process in such a way that the educational-dominant theory and practice of developmental education will focus on methodologies and methods of self-development of the individuals, on the one hand, and the program of expanded professional (psychological) support of self-education of the individuals, on the other hand. The purpose of the article is to determine the update on the problem of tutors’ psychological readiness for professional activity in the system of higher education of Ukraine. The analysis of the respondents’ answers, and the comparison of the results of teachers training for the tutor activity at the start and end of the experimental work give grounds to fix quantitative and qualitative changes in the formation of psychological readiness for tutor activity in teachers. The level of future tutors’ psychological readiness for the tutor activity in the experimental and control groups at the formative stage of the experiment has been increased due to a qualitatively prepared certification program «professional tutors training»; active participation in practical trainings including discussions, round tables, thematic conferences devoted to introduction of excellence tutoring; and conducting training exercises for the development of innovative skills, research and experimental work..