Employee Turnover has been considered as one of the main concerning issues in any organization. Therefore, a lot of examinations have been conducted to identify the factors influencing the Employee Turnover. In the studies done earlier, the Organizational Culture as a determinant of the Employee Turnover has not been much taken into consideration. In this regard, the present study aimed to uncover the relationship between the Organizational Culture and the Employee Turnover. Data from people working in different industries were collected through a structured close-ended questionnaire. The regression model was used to determine the relationship between Organizational Culture and Employee Turnover. The Organizational Culture’s Dimensions highlighted by previous studies were used to test the relationship. Furthermore, these factors of the Organizational Culture were separately tested against the Employee Turnover to see the significance of each factor. On this basis, new model has been suggested. Generally, the current examination indicated that the Organizational Culture had a remarkable influence on the Employee Turnover, and there was an inverse relationship between the Organizational Culture and the Employee Turnover.