Introduction: Fire is one of the most dangerous phenomena that causing serious financial damages and casualties. Hospital fires are a major problem due to the presence of disabled and sensitive patients, lack of public awareness, access to fire extinguisher equipment, access to escape routes, and so on. Evaluation of fire safety management is essential to identify measures to prevent fire and promotion of fire safety. Objective: The objective of this study is to evaluate the status of fire safety management in order to prioritize measures and promotion of fire safety in the building of selected hospitals in Tehran and Tabriz. Methods: This cross-sectional descriptive-analytical study was conducted in 10 selected hospital buildings of Tehran and Tabriz. Research tools included interviews with health and safety representatives and a questionnaire presented by L.T. Wong in the form of fire safety evaluation (FSE) checklist and based on localization conditions translated. The questionnaire consists of 4 categories: First: building specifications and features (5 items), Second: fire service installation and implementation (10 items); Third: fire safety management (19 items) and Fourth: fire safety maintenance and repair (9 items). By using checklists and formulas of mean item scores, the score of achieving fire safety level for each category and building score obtained as well as overall fire safety level of hospital buildings. Results: The status of fire safety management in selected hospitals of Tehran and Tabriz by using FSE checklist shows that building score, which indicates the level of overall fire safety of hospital building is about 55.87%. conclusion: Given the fire safety level status, which is below 60% and interviews with hospital health and safety representatives, it was determined that in most of the studied hospital buildings, there is no ramps or slope routes for patients as well as automatic fire extinguishing systems or sprinklers and smoke stop doors, and there is no regular and periodic inspection and maintenance of fire extinguishing equipment.