The role of jewelry in the Safavid Era has been prominent in the northwest of Iran. In this era, jewelry has been used as decorative elements in architecture, jewelry-making, and enameling. The motifs of these jewelry are inspired by the Islamic-Iranian art. The present study, which is conducted using the library method, has archaeologically investigated the jewelry inlaid with precious and semi-precious stones in the Safavid Era in Ardabil City. The results indicated that the stones most used in the Safavid Era included ruby, turquoise, and agate. Turquoise and agate were mined in Iran, and ruby was imported from foreign countries by traders and merchants. The evaluations indicate that the jewelry was decorated by motifs such as floral, geometric, plant, and vegetal motifs. These decorations were mostly carved on silver in the form of toreutics and tapestry. Also, in some cases, the body parts motifs such as hands and eyes have been used. The samples of the present study include necklaces composed of precious metals, silver, and precious and semi-precious stones such as ruby. Ruby was more of a divine symbol and has been used as a stone of bravery and love. The agate has been used as a protecting stone, and turquoise has been a symbol of peace, purity, and transparency, which were often inlaid in the center of jewelry. It was believed that these stones had transcendental powers that protected the owner from threats and kept him safe from dangers. The Safavid art jewelry enjoyed exquisite and beautiful details inspired by religious symbols. They were not only used as ornaments and decorations but also as symbols of the prominent Safavid art and design which were rooted in Sufism. They were also known as elements of the cultural and religious identity of the Safavid.